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Review of Scientific Instruments / Volume 82 / Issue 8 / NOTES

Rev. Sci. Instrum. 82, 086102 (2011); http://dx.doi.org/10.1063/1.3623502 (3 pages)

Note: Direct force and ionic-current measurements on DNA in a nanocapillary

O. Otto, L. J. Steinbock, D. W. Wong, J. L. Gornall, and U. F. Keyser

Cavendish Laboratory, University of Cambridge, CB3 0HE Cambridge, United Kingdom

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(Received 29 April 2011; accepted 19 July 2011; published online 10 August 2011)

We have developed optical tweezers, with force measurements based on fast video tracking, for analysis and control of DNA translocation through nanocapillaries. Nanocapillaries are single-molecule biosensors with very similar characteristics to solid-state nanopores. Our novel experimental setup allows for ionic-current measurements in which the nanocapillary is oriented perpendicular to the trapping laser. Using video-based particle tracking, we are able to measure the position of DNA coated colloids at sub-millisecond resolution and in real-time. We present the first electrophoretic force and simultaneous ionic-current measurements of a single DNA molecule inside the orifice of a nanocapillary.

© 2011 American Institute of Physics

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KEYWORDS and PACS

Keywords

biomechanics, biosensors, DNA, force measurement, molecular biophysics, nanobiotechnology, nanostructured materials, orifices (mechanical), radiation pressure

PACS

  • 87.85.G-

    Biomechanics

  • 07.10.-h

    Mechanical instruments and equipment

  • 87.15.-v

    Biomolecules: structure and physical properties

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.3623502

PUBLICATION DATA

ISSN

0034-6748 (print)  
1089-7623 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
    A. Ashkin, Phys. Rev. Lett. 24, 598 (1970).


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