RSI Nameplate
  • Volume/Page
  • Keyword
  • DOI
  • Citation
  • Advanced
   
 
 
 

Flickr Twitter iResearch App Facebook

BROWSE VOLUMES

Year Range: 
Search Issue | RSS Feeds RSS
Previous Issue Next Issue

Aug 2009

Volume 80, Issue 8, Articles (08xxxx)

Issue Cover Spotlight Figure
Return to All Sections
back to top
RSS Feeds

Invited Review Article: Imaging techniques for harmonic and multiphoton absorption fluorescence microscopy

Ramón Carriles, Dawn N. Schafer, Kraig E. Sheetz, Jeffrey J. Field, Richard Cisek, Virginijus Barzda, Anne W. Sylvester, and Jeffrey A. Squier

Rev. Sci. Instrum. 80, 081101 (2009); http://dx.doi.org/10.1063/1.3184828 (23 pages) | Cited 26 times

Online Publication Date: 5 August 2009

Full Text: Read Online (HTML) | Download PDF

Show Abstract
We review the current state of multiphoton microscopy. In particular, the requirements and limitations associated with high-speed multiphoton imaging are considered. A description of the different scanning technologies such as line scan, multifoci approaches, multidepth microscopy, and novel detection techniques is given. The main nonlinear optical contrast mechanisms employed in microscopy are reviewed, namely, multiphoton excitation fluorescence, second harmonic generation, and third harmonic generation. Techniques for optimizing these nonlinear mechanisms through a careful measurement of the spatial and temporal characteristics of the focal volume are discussed, and a brief summary of photobleaching effects is provided. Finally, we consider three new applications of multiphoton microscopy: nonlinear imaging in microfluidics as applied to chemical analysis and the use of two-photon absorption and self-phase modulation as contrast mechanisms applied to imaging problems in the medical sciences.
Show PACS
42.65.Ky Frequency conversion; harmonic generation, including higher-order harmonic generation
42.62.Fi Laser spectroscopy
42.65.Jx Beam trapping, self-focusing and defocusing; self-phase modulation
Return to All Sections
Close

Google Calendar

ADVERTISEMENT

Go to AIP Home   © AIP Publishing LLC
close