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Review of Scientific Instruments / Volume 79 / Issue 10 / MICROSCOPY AND IMAGING

Rev. Sci. Instrum. 79, 103705 (2008); http://dx.doi.org/10.1063/1.2999542 (5 pages)

Optical trapping and surgery of living yeast cells using a single laser

Jun Ando1,2, Godofredo Bautista1, Nicholas Smith2, Katsumasa Fujita2, and Vincent Ricardo Daria1

1National Institute of Physics, University of the Philippines, Quezon City, 1101 Philippines
2Department of Applied Physics, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan

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(Received 8 June 2008; accepted 12 September 2008; published online 20 October 2008)

We present optical trapping and surgery of living yeast cells using two operational modes of a single laser. We used a focused laser beam operating in continuous-wave mode for noninvasive optical trapping and manipulation of single yeast cell. We verified that such operational mode of the laser does not cause any destructive effect on yeast cell wall. By changing the operation of the laser to femtosecond-pulsed mode, we show that a tightly focused beam dissects the yeast cell walls via nonlinear absorption. Lastly, using the combined technique of optical microsurgery and trapping, we demonstrate intracellular organelle extraction and manipulation from a yeast cell. The technique established here will be useful as an efficient method for both surgery and manipulation of living cells using a single laser beam.

© 2008 American Institute of Physics

Article Outline

  1. INTRODUCTION
  2. EXPERIMENTAL PROCEDURE
  3. OPTICAL MANIPULATION OF SINGLE YEAST CELLS
  4. OPTICAL SURGERY OF YEAST CELLS
  5. COMBINING OPTICAL TRAPPING AND SURGERY FOR EXTRACTING CELL ORGANELLES
  6. CONCLUSION

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KEYWORDS and PACS

Keywords

cellular biophysics, high-speed optical techniques, laser applications in medicine, microorganisms, radiation pressure, surgery

PACS

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.2999542

PUBLICATION DATA

ISSN

0034-6748 (print)  
1089-7623 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
    N. I. Smith, K. Fujita, T. Kaneko, K. Katoh, O. Nakamura, S. Kawata, and T. Takamatsu, Appl. Phys. Lett. 79, 1208 (2001)APPLAB000079000008001208000001.


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