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Review of Scientific Instruments / Volume 78 / Issue 4 / BIOLOGY AND MEDICINE

Rev. Sci. Instrum. 78, 044301 (2007); http://dx.doi.org/10.1063/1.2722400 (5 pages)

Easily fabricated magnetic traps for single-cell applications

John H. Koschwanez, Robert H. Carlson, and Deirdre R. Meldrum

Department of Electrical Engineering, University of Washington, Seattle, Washington 98195-2500

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(Received 8 January 2007; accepted 11 March 2007; published online 17 April 2007)

We describe a simple and inexpensive method of fabricating single cell magnetic traps within a polydimethylsiloxane (PDMS) device. These traps were developed as part of an automated system that captures individual yeast cells in a microfluidic device and analyzes each cell as it buds. To make the traps, PdCl2 catalyst is rubbed with vinyl foam onto plasma-patterned PDMS, and then Co-Ni-B alloy is electrolessly deposited onto the catalyst at a moderate temperature. We demonstrate individual yeast cell capture and estimate the capture force (1.9−4.4 pN) by measuring the flow speed required to remove the cell from its trap in a microfluidic channel.

© 2007 American Institute of Physics

Article Outline

  1. INTRODUCTION
  2. TRAPPING DEVICE FABRICATION
  3. MAGNETIC TRAP CHARACTERIZATION
    1. Measurement of element height
    2. Estimation of trapping force
  4. DISCUSSION

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KEYWORDS and PACS

Keywords

magnetic traps, biomagnetism, cellular biophysics, microchannel flow, bioMEMS, biological techniques, palladium compounds, polymers, cobalt alloys, nickel alloys, boron alloys

PACS

  • 87.80.-y

    Biophysical techniques (research methods)

  • 87.17.-d

    Cell processes

  • 47.60.-i

    Flow phenomena in quasi-one-dimensional systems

  • 47.85.Np

    Fluidics

  • 85.85.+j

    Micro- and nano-electromechanical systems (MEMS/NEMS) and devices

  • 07.10.Cm

    Micromechanical devices and systems

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.2722400

PUBLICATION DATA

ISSN

0034-6748 (print)  
1089-7623 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
    S. K. Sia and G. M. Whitesides, Electrophoresis 24, 3563 (2003)JAPIAU000044000009004209000001.

    J. H. P. Watson, J. Appl. Phys. 44, 4209 (1973)JAPIAU000044000009004209000001.

    E. Mirowski, J. Moreland, S. E. Russek, and M. J. Donahue, Appl. Phys. Lett. 84, 1786 (2004)APPLAB000084000010001786000001.

    H. Lee, A. M. Purdon, and R. M. Westervelt, Appl. Phys. Lett. 85, 1063 (2004)APPLAB000085000006001063000001.

    V. Studer, G. Hang, A. Pandolfi, M. Ortiz, W. F. Anderson, and S. R. Quake, J. Appl. Phys. 95, 393 (2004)JAPIAU000095000001000393000001.

    D. W. Inglis, R. Riehn, R. H. Austin, and J. C. Sturm, Appl. Phys. Lett. 85, 5093 (2004)APPLAB000085000021005093000001.


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