Jump to Content
Your Recent History
View Cart
LOG IN or SELECT A PURCHASE OPTION:
Rev. Sci. Instrum. 74, 4012 (2003); http://dx.doi.org/10.1063/1.1599062 (9 pages)
Oscillatory magnetic tweezers based on ferromagnetic beads and simple coaxial coils
(Received 12 March 2003; accepted 6 June 2003)
Alerts
Alert Me When Cited
Alert Me When Corrected
Tools
Download Citation
Add to MyScitation
Permissions / Reprints
Blog This Article
Print-Friendly
Research Toolkit
Share
Email Abstract
Connotea
CiteULike
del.icio.us
BibSonomy
Tweet this Article
Add to FacebookWe report the design and validation of simple magnetic tweezers for oscillating ferromagnetic beads in the piconewton and nanometer scales. The system is based on a single pair of coaxial coils operating in two sequential modes: permanent magnetization of the beads through a large and brief pulse of magnetic field and generation of magnetic gradients to produce uniaxial oscillatory forces. By using this two step method, the magnetic moment of the beads remains constant during measurements. Therefore, the applied force can be computed and varies linearly with the driving signal. No feedback control is required to produce well defined force oscillations over a wide bandwidth. The design of the coils was optimized to obtain high magnetic fields (280 mT) and gradients (2 T/m) with high homogeneity (5% variation) within the sample. The magnetic tweezers were implemented in an inverted optical microscope with a videomicroscopy-based multiparticle tracking system. The apparatus was validated with 4.5 μm magnetite beads obtaining forces up to ∼2 pN and subnanometer resolution. The applicability of the device includes microrheology of biopolymer and cell cytoplasm, molecular mechanics, and mechanotransduction in living cells. © 2003 American Institute of Physics.
© 2003 American Institute of Physics
RELATED DATABASES
To view database links for this article,
you need to log in.
KEYWORDS and PACS
Keywords
ARTICLE DATA
Digital Object Identifier
For access to fully linked references, you need to log in.
-
D. Wirtz, Phys. Rev. Lett. 75, 2436 (1995).
F. Amblard, A. C. Maggs, B. Yurke, A. Pargellis, and S. Leibler, Phys. Rev. Lett. 77, 4470 (1996).
B. Fabry, G. N. Maksym, J. P. Butler, M. Glogauer, D. Navajas, and J. J. Fredberg, Phys. Rev. Lett. 87, 148102 (2001).
G. Binnig, C. F. Quate, and C. Gerber, Phys. Rev. Lett. 56, 930 (1986).
A. Ashkin and J. M. Dziedzic, Phys. Rev. Lett. 54, 1245 (1985).
F. Assi, R. Jenkins, J. Yang, C. Love, and M. Prentiss, J. Appl. Phys. 92, 5584 (2002)JAPIAU000092000009005584000001.
M. Keller, J. Schilling, and E. Sackmann, Rev. Sci. Instrum. 72, 3626 (2001)RSINAK000072000009003626000001.
C. Haber and D. Wirtz, Rev. Sci. Instrum. 71, 4561 (2000)RSINAK000071000012004561000001.
F. Amblard, B. Yurke, A. Pargellis, and S. Leibler, Rev. Sci. Instrum. 67, 818 (1996)RSINAK000067000003000818000001.
P. N. Murgatroyd and B. E. Bernard, Rev. Sci. Instrum. 54, 1736 (1983)RSINAK000054000012001736000001.
M. Rudd and J. Craig, Rev. Sci. Instrum. 39, 1372 (1968)RSINAK000039000009001372000001.
R. Cacak and J. Craig, Rev. Sci. Instrum. 40, 1468 (1969)RSINAK000040000011001468000001.
K. Kaminishi and S. Nawata, Rev. Sci. Instrum. 52, 447 (1981)RSINAK000052000003000447000001.
J. Wang, S. She, and S. Zhang, Rev. Sci. Instrum. 73, 2175 (2002)RSINAK000073000005002175000001.
R. A. J. Schill and K. Hoff, Rev. Sci. Instrum. 72, 2769 (2001)RSINAK000072000006002769000001.
F. Gittes, B. Schnurr, P. D. Olmsted, F. C. MacKintosh, and C. F. Schmidt, Phys. Rev. Lett. 79, 3286 (1997).
F. G. Schmidt, B. Hinner, and E. Sackmann, Phys. Rev. E 61, 5646 (2000).
F. G. Schmidt, B. Hinner, E. Sackmann, and J. X. Tang, Phys. Rev. E 62, 5509 (2000).
For access to citing articles, you need to log in.
This Publication
Scitation
SPIN
Google Scholar
PubMed