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Jun 1999

Volume 70, Issue 6, pp. 2567-2883

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back to top BIOLOGY and MEDICINE

Miniaturized scintillation technique for protein solubility determinations

Angela R. Feeling-Taylor, R. Michael Banish, Rhoda Elison Hirsch, and Peter G. Vekilov

Rev. Sci. Instrum. 70, 2845 (1999); http://dx.doi.org/10.1063/1.1149805 (5 pages) | Cited 5 times

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We have developed a miniaturized (volume of crystallizing solution ∼100 μl) technique for the determination of protein solubility as a function of temperature. After nucleation, crystals are detected by the light they scatter. Then the temperature at which a solution with the initial concentration is in equilibrium with the crystals is sought by stepwise, equilibrium dissolution of the crystals. The approach to solubility from the side of dissolution provides for higher accuracy of the determinations. The method was used to determine the temperature dependence of the solubility of human hemoglobin (Hb) C, for which high-resolution x-ray crystallography data are needed to understand the structural basis for the drastically different in vivo aggregation/crystallization behavior of β6 Hb mutants. © 1999 American Institute of Physics.
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87.15.N- Properties of solutions of macromolecules
87.14.E- Proteins
87.64.Cc Scattering of visible, uv, and infrared radiation
87.15.M- Spectra of biomolecules

Teeth mobility measurement by laser Doppler vibrometer

P. Castellini and L. Scalise

Rev. Sci. Instrum. 70, 2850 (1999); http://dx.doi.org/10.1063/1.1149806 (6 pages) | Cited 3 times

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In this work a new procedure for the assessment of teeth mobility, based on a noncontact sensor (laser Doppler vibrometer), is presented. The procedure is based on the manual application of impulses applied with an instrumented hammer on the tooth crown. The ratio of the maximum displacement of the tooth to the peak of the input force has been considered as the mobility degree index. A basic mechanical model of the hammer-tooth impact based on the data extracted from literature has been realized. Results produced with the model mentioned above have been compared with those measured with in vitro and in vivo tests using the proposed procedure. Good agreement between data from the mechanical model and in vitro conditions has been found. Tests carried out in in vitro and in vivo conditions have demonstrated the validity of the procedure proposed. Measurement results compared to those found in the relevant literature show the validity of the procedure proposed. © 1999 American Institute of Physics.
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87.63.L- Visual imaging
87.19.R- Mechanical and electrical properties of tissues and organs
42.62.Be Biological and medical applications
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