A method to track rotational motion for use in single-molecule biophysics
Jan Lipfert, Jacob J. W. Kerssemakers, Maylon Rojer, and Nynke H. Dekker
Magnetic tweezers (MTs) are a single-molecule technique that enables the application of precisely calibrated stretching forces to nucleic acid tethers and to control their rotational motion. However, conventional magnetic tweezers do not directly monitor rotation or measure torque. Here, a method to directly measure rotational motion of particles in MT is described.